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KMID : 0361020080510030240
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Korean Journal of Otolaryngology - Head and Neck Surgery
2008 Volume.51 No. 3 p.240 ~ p.249
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Effects of Repeated Ozone Exposure on Airway Remodeling in Mice
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Kim Yong-Min
Kim Ah-Young
Rha Ki-Sang
Kim Hyo-Seon
Han Wook-Kyoung
Kim In-Jung
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Abstract
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Background & Objectives: To investigate whether repeated long-term exposure of ozone has an effect on airway remodeling and whether ozone exposure has an aggravating effect on airway remodeling during allergen challenge in previously sensitized mice.
Materials & Method: Forty female BALB/c mice were divided into four groups as follows: Group I as the control group, Group II the ovalbumin aerosol exposure group, Group III the ozone exposure group, and Group IV the ovalbumin aerosol and ozone exposure group. Twenty-four hours after the last OVA challenge, the concentration of IFN-gamma, IL-5, IL-13 and TGF-beta and the count of eosinophil and lymphocyte were measured in nasal lavage fluid (NLF) and bronchoalvelolar lavage fluid (BALF). Immunohistochemical staining for MMP-9 and TIMP-1 were performed. Trichrome staining was also conducted to evaluate subepithelial fibrosis.
Results: Group II and III exhibited increased IL-5, IL-13, and TGF-beta in NLF and BALF, compared to Group I;Group IV showed more increased pattern for these cytokines, compared to Group II and III. Group II, III, and IV also showed increased expression of MMP-9 and TIMP-1, compared to Group I in lung;Group II and IV showed more compared to Group I and III in nasal cavity mucosa. Subepithelial fibrosis was prominent in Group II, III, and IV, compared with group I. In group IV, subepithelial fibrosis was more increased than Group II and III.
Conclusions: These results suggest that ozone exposure induces remodeling in upper and lower airway and also enhances allergen-mediated airway remodeling in previously sensitized animals.
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KEYWORD
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Remodeling, Ozone, Allergic rhinitis
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